Journal: Cell death & disease

Article Title: Deletion of Mettl3 in mesenchymal stem cells promotes acute myeloid leukemia resistance to chemotherapy.

doi: 10.1038/s41419-023-06325-7

Figure Lengend Snippet: Fig. 1 Deletion of Mettl3 in MSCs induces high marrow adiposity in mice. A Western blot analysis of METTL3 in BMMSCs isolated from mice. B Detections of m6A levels in total RNA in BMMSCs from mice using EpiQuik m6A RNA Methylation Quantification Kits. C Representative images of H&E staining and FABP4 immunohistochemical staining of the distal femora from mice. Scale Bars: 100 μm. D Quantification of adipocyte number and area per tissue area in the distal marrow (n = 8). E ELISA examined the contents of supernatant adipokines in bone marrow rinses of mice (n = 6). F Representative images and quantitative analyses of Alizarin Red Solution (ARS) staining of BMMSCs isolated from mice. Scale Bars: 200 μm. G Representative images and quantitative analyses of ORO staining of BMMSCs isolated from mice. Scale Bars: 200 μm. The data are presented as mean ± SD. The two-tailed unpaired Student’s t test was used to compare two groups and one-way ANOVA with Dunnett’s multiple comparisons test was used for data with more than two groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns not significant.

Article Snippet: In the supernatant, based on the Cell Death and Disease (2023) 14:796 manufacturer’s directions, the levels of adipokines, like resistin, leptin, and growth hormone, were quantified employing the kits of Mouse Resistin ELISA (Cat# CSB-E06886m, CUSABIO, Wuhan, Hubei, China), Mouse Leptin ELISA (Cat# CSB-E04650m, CUSABIO), and Mouse Growth Hormone ELISA (Cat# CSB-E07343m, CUSABIO), respectively.

Techniques: Western Blot, Isolation, Methylation, Staining, Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Cell death & disease

Article Title: Deletion of Mettl3 in mesenchymal stem cells promotes acute myeloid leukemia resistance to chemotherapy.

doi: 10.1038/s41419-023-06325-7

Figure Lengend Snippet: Fig. 4 METTL3 deletion reduces AML chemosensitivity by inducing adipogenic differentiation of OP9 cells. A Representative images and quantification of ORO staining in Mettl3 overexpression cells after 14 days of adipogenic induction. Scale bar, 100 µm. B qPCR analysis of the expression of adipogenic marker genes, Adipoq, Cebpa, Lpl, Plin1, CD36, and Pparγ in Mettl3 overexpression cells under adipogenic conditions. C ORO staining of Mettl3 knockdown cells following adipogenic induction. Scale bar, 100 µm. Absorbance at OD500 was determined for ORO staining in isopropanol at room temperature. D qPCR analysis of adipogenic lineage–associated gene expression after induction of differentiation in Mettl3 knockdown cells. E ELISA detection of adipokine concentration in the culture medium before induction of differentiation and on day 14 of Mettl3 overexpression cells. F ELISA detection of adipokine concentration in the culture medium before induction of differentiation and on day 14 of Mettl3 knockdown cells. G After adipogenic differentiation of Mettl3 overexpression cells, AML cells were co-cultured with them for 24 h to test their chemoresistance to Ara-C and DNR. H After adipogenic differentiation of Mettl3 knockdown cells, AML cells were co-cultured with them for 24 h to test their chemoresistance to Ara-C and DNR. Blank, blank control; OE-NC, negative control of overexpression; Mettl3-OE, overexpression of mouse Mettl3; sh-NC, negative control of knockdown; shMettl3-1 and shMettl3-2, independent shRNAs targeting mouse Mettl3. The data are presented as mean ± SD. The two-tailed unpaired Student’s t test was used to compare two groups and one-way ANOVA with Dunnett’s multiple comparisons test was performed for multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns not significant.

Article Snippet: In the supernatant, based on the Cell Death and Disease (2023) 14:796 manufacturer’s directions, the levels of adipokines, like resistin, leptin, and growth hormone, were quantified employing the kits of Mouse Resistin ELISA (Cat# CSB-E06886m, CUSABIO, Wuhan, Hubei, China), Mouse Leptin ELISA (Cat# CSB-E04650m, CUSABIO), and Mouse Growth Hormone ELISA (Cat# CSB-E07343m, CUSABIO), respectively.

Techniques: Staining, Over Expression, Expressing, Marker, Knockdown, Gene Expression, Enzyme-linked Immunosorbent Assay, Concentration Assay, Cell Culture, Control, Negative Control, Two Tailed Test

Journal: Cell death & disease

Article Title: Deletion of Mettl3 in mesenchymal stem cells promotes acute myeloid leukemia resistance to chemotherapy.

doi: 10.1038/s41419-023-06325-7

Figure Lengend Snippet: Fig. 6 AKT inhibitor MK2206 mediates the adipogenesis level of OP9 cells. A Western blot analysis of AKT and p-AKT1 (Ser473) in OP9 cells treated with MK2206, an AKT inhibitor. B ORO staining of DMSO and MK2206 treated cells after adipo-induced for 14 days. Scale bar, 100 μm. C ELISA detected the concentration of adipokines in the culture medium before induction of differentiation and on day 14 under treatment with MK2206. D After being treated with MK2206 for 18 h and adipo-induced for 14 days, co-culturing with AML cells for 24 h was performed to test the chemoresistance of AML cells to Ara-C and DNR. The data are presented as mean ± SD. The two-tailed unpaired Student’s t test was used to compare two groups and one-way ANOVA with Dunnett’s multiple comparisons test was performed for multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001, ns not significant.

Article Snippet: In the supernatant, based on the Cell Death and Disease (2023) 14:796 manufacturer’s directions, the levels of adipokines, like resistin, leptin, and growth hormone, were quantified employing the kits of Mouse Resistin ELISA (Cat# CSB-E06886m, CUSABIO, Wuhan, Hubei, China), Mouse Leptin ELISA (Cat# CSB-E04650m, CUSABIO), and Mouse Growth Hormone ELISA (Cat# CSB-E07343m, CUSABIO), respectively.

Techniques: Western Blot, Staining, Enzyme-linked Immunosorbent Assay, Concentration Assay, Two Tailed Test

Journal: bioRxiv

Article Title: High-fat diet exacerbates the loss of bone fracture toughness in aging for C57BL/6JN mice

doi: 10.1101/2024.08.13.607633

Figure Lengend Snippet: Levels of inflammatory biomarkers: (a) leptin, (b) resistin, (c) vascular endothelial growth factor (VEGF), (d) C-reactive protein (CRP) (e) adipsin, (f) adiponectin, (g) fibroblast growth factor 21 (FGF21), (h) interleukin 4 (IL-4), (i) IL-10, and (j) IL-6. Boxplots illustrate the interquartile range (box), the minimum and maximum values (whiskers), the median value (bar across the box), the mean value (white square), and individual data points (black circles). Significant p-values from 3-way ANOVA and pairwise post-hoc tests are noted on the plots. For all models, the glucose area under the curve (AUC) was considered as a covariate. If the covariate was not significant, the model was rerun without it.

Article Snippet: Measurements included CTX1 (Mouse Cross Linked C-telopeptide of Type I collagen Mini Samples ELISA kit, MyBioSource, San Diego, CA), P1NP ( Mouse procollagen I N-terminal peptide, P1NP ELISA kit (MyBioSource, San Diego, CA), adiponectin (Quantikine ELISA Mouse Adiponectin/Acrp30 kit, R&D Systems, Minneapolis, MN), resistin (Quantikine ELISA Mouse Resistin kit (R&D Systems, Minneapolis, MN), and interleukins 4, 6, and 10 (IL-4, IL-6, IL-10), fibroblast growth factor 21 (FGF21), leptin, vascular endothelial growth factor (VEGF), C-reactive protein (CRP), and complement factor D/adipsin (CFD/adipsin) (Luminex Discovery Assay Mouse Premixed Multi-Analyte kits, R&D Systems, Minneapolis, MN).

Techniques:

Journal: Theranostics

Article Title: NAD + -boosting therapy alleviates nonalcoholic fatty liver disease via stimulating a novel exerkine Fndc5/irisin

doi: 10.7150/thno.53652

Figure Lengend Snippet: Nicotinamide riboside, a well-established NAD+-boosting molecule, stimulates Fndc5/irisin in murine and human. (A-F) The influence of NR treatment (400 mg/kg/d) on plasma concentrations of FGF1, FGF21, resistin, and adiponectin in HFD-induced NAFLD mouse model. FGF1, FGF21, resistin and adiponectin plasma concentrations were changed in NAFLD mice but not by NR. ( E ) Plasma irisin concentration was changed by NR treatment. ** P <0.01 vs Chow, ## P <0.01 vs HFD, n = 8. NS, no significance. Two different commercial ELISA kits from Phoenix Pharmaceutical (Manufacturer P) and AdipoGen (Manufacturer A) were used. (F) The protein expression of Fndc5 in skeletal muscle of NAFLD mice with or without NR treatment. ** P <0.01 vs Chow, ## P <0.01 vs HFD, n = 4. (G) The protein expression of Fndc5 in other tissues such as liver and adipose of NAFLD mice with or without NR treatment. ** P <0.01 vs HFD by unpaired t -test, n = 4. (H-I) The influences of two-week NR supplement (500 mg, bid) or physical exercise on plasma irisin concentration in human volunteers detected by two different commercial ELISA kits from Phoenix Pharmaceutical (Manufacturer P, H ) and AdipoGen (Manufacturer A, I ). * P <0.05, ** P <0.01 by paired t -test, n = 6.

Article Snippet: The plasma concentrations of fibroblast growth factor-1 (FGF1, Abcam, Cambridge, UK, Catalogue: DY4686-05), FGF21 (R&D Biosystems, Minneapolis, MN, Catalogue: MF2100), resistin (Boster Biological, Huhan, China, Catalogue: EK0582), adiponectin (R&D Biosystems, Catalogue: MRP300), leptin (R&D Biosystems, Minneapolis, MN, Catalogue: MOB00), chemerin (Boster Biological, Huhan, China, Catalogue: EK0582), vaspin (Aviscera Bioscience, Santa Clara, CA), hepcidin (Bioss Biotechnology Co., Beijing, China, Catalogue: bsk00526), interleukin-6 (IL-6, R&D Biosystems, Minneapolis, MN, Catalogue: D6050) and C-X-C motif chemokine 10 (CXCL10, PeproTech, Rocky Hill, NJ, Catalogue: 900-K) were detected using commercial ELISA kits according to the manufacturer's instructions.

Techniques: Clinical Proteomics, Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Biomedicines

Article Title: Disease-Modifying Potential of Metformin and Alendronate in an Experimental Mouse Model of Osteoarthritis

doi: 10.3390/biomedicines9081017

Figure Lengend Snippet: Serum resistin ( A ) and leptin ( B ) levels at day 30 of CIOA. Data are means ± SD from three determinations ( n = 10 per group). * p < 0.05, ** p < 0.01, *** p < 0.001, one-way ANOVA. (CIOA = collagenase-induced osteoarthritis; metf = metformin; alendr = alendronate).

Article Snippet: The concentration of resistin in sera was quantified by the Mouse Resistin (RETN) ELISA kit (Abbexa Ltd., Cambridge, UK) with a sensitivity of 46 pg/mL, and leptin was quantified by mouse ELISA kit (BioVendor, Brno, Czech Republic) with a sensitivity of 30 pg/mL.

Techniques: